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vector encoding bcl 2 protein wild type  (Addgene inc)


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    Structured Review

    Addgene inc vector encoding bcl 2 protein wild type
    Vector Encoding Bcl 2 Protein Wild Type, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+encoding+bcl+2+protein+wild+type/GFP-Bcl2+(Plasmid+%2317999)/pm23836782-28-56-61
    Average 92 stars, based on 27 article reviews
    vector encoding bcl 2 protein wild type - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Stable Transfection:

    Article Title: BH4 domain of bcl-2 protein is required for its proangiogenic function under hypoxic condition.
    Article Snippet: Cell cultures, transfections and treatments Human umbilical endothelial cells (HUVEC; PromoCell GmbH, Heidelberg, Germany) and human melanoma cell lines (M14 and A375SM-SC1) were cultured as previously reported (14). .. M14 and A375SM-SC1 cells were stably transfected using Lipofectamine (Invitrogen, Carlsbad, CA) with expression vectors encoding the human bcl-2 protein wild-type or deleted of BH4 domain (aa 1–36), or mutated in BH1 (G145E) or BH2 (W188A) domains (13), with Flag-tagged vector encoding the only BH4 domain of bcl-2 (aa 1–30) or with green fluorescent protein (GFP)-tagged vector encoding bcl-2 protein wild-type (Addgene plasmid 17999) or targeted to ER (Addgene plasmid 18000). ..

    Transfection:

    Article Title: BH4 domain of bcl-2 protein is required for its proangiogenic function under hypoxic condition.
    Article Snippet: Cell cultures, transfections and treatments Human umbilical endothelial cells (HUVEC; PromoCell GmbH, Heidelberg, Germany) and human melanoma cell lines (M14 and A375SM-SC1) were cultured as previously reported (14). .. M14 and A375SM-SC1 cells were stably transfected using Lipofectamine (Invitrogen, Carlsbad, CA) with expression vectors encoding the human bcl-2 protein wild-type or deleted of BH4 domain (aa 1–36), or mutated in BH1 (G145E) or BH2 (W188A) domains (13), with Flag-tagged vector encoding the only BH4 domain of bcl-2 (aa 1–30) or with green fluorescent protein (GFP)-tagged vector encoding bcl-2 protein wild-type (Addgene plasmid 17999) or targeted to ER (Addgene plasmid 18000). ..

    Expressing:

    Article Title: BH4 domain of bcl-2 protein is required for its proangiogenic function under hypoxic condition.
    Article Snippet: Cell cultures, transfections and treatments Human umbilical endothelial cells (HUVEC; PromoCell GmbH, Heidelberg, Germany) and human melanoma cell lines (M14 and A375SM-SC1) were cultured as previously reported (14). .. M14 and A375SM-SC1 cells were stably transfected using Lipofectamine (Invitrogen, Carlsbad, CA) with expression vectors encoding the human bcl-2 protein wild-type or deleted of BH4 domain (aa 1–36), or mutated in BH1 (G145E) or BH2 (W188A) domains (13), with Flag-tagged vector encoding the only BH4 domain of bcl-2 (aa 1–30) or with green fluorescent protein (GFP)-tagged vector encoding bcl-2 protein wild-type (Addgene plasmid 17999) or targeted to ER (Addgene plasmid 18000). ..

    Plasmid Preparation:

    Article Title: BH4 domain of bcl-2 protein is required for its proangiogenic function under hypoxic condition.
    Article Snippet: Cell cultures, transfections and treatments Human umbilical endothelial cells (HUVEC; PromoCell GmbH, Heidelberg, Germany) and human melanoma cell lines (M14 and A375SM-SC1) were cultured as previously reported (14). .. M14 and A375SM-SC1 cells were stably transfected using Lipofectamine (Invitrogen, Carlsbad, CA) with expression vectors encoding the human bcl-2 protein wild-type or deleted of BH4 domain (aa 1–36), or mutated in BH1 (G145E) or BH2 (W188A) domains (13), with Flag-tagged vector encoding the only BH4 domain of bcl-2 (aa 1–30) or with green fluorescent protein (GFP)-tagged vector encoding bcl-2 protein wild-type (Addgene plasmid 17999) or targeted to ER (Addgene plasmid 18000). ..



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